Reproductive biotechnology applied to the farm animals

Research Area (Heading): LARGE ANIMALS PHYSIOLOGY, REPRODUCTION AND MEDICINE

Academic field (SSD): Clinical veterinary obstetrics and gynecology

Contact person:

Dr. Calogero Stelletta

Dept. of Animal Medicine, Production and Health

Viale dell'Università 16 35020 Legnaro, Padova, Italy

Tel.:+39-049-8272791 Fax: +39-049-8272954

Email: calogero.stelletta@unipd.it

People involved in the Research Topic: Prof. Stefano Romagnoli, Prof. Antonio Mollo, Dr. Calogero Stelletta, Dr.ssa Donatella Gelli, Dr.ssa Chiara Milani, Dr.ssa Maria Elena Falomo

Description:

The research topic is related to the optimization of the use of inseminating dose in farm animals. Ultrasonography (B-mode and Doppler), thermography, semen analysis, seminal plasma composition, natural fluids composition and semen filtering are the main diagnostic tools integrated in the innovative approach to obtain a higher reproductive efficiency in farm animals (Bovine, Ovine, Caprine, Camelids, Equine). The same approach is considerable also in the case of breed/species preservation when they are endangered.

SPECIFIC PROJECT CALL 2019

Optimization of the use of the artificial insemination dose in farm animals

The selection pressure which may be improved in an animal population depends by the breeders selected. Breeders can be considered only the male’s population or also the females. When both the sexes are selected as breeders the “pressure” impressed to the population is amplified and it can be possible to obtain a higher “response” in terms of selection targets. The classical means to impress the higher pressure are the artificial insemination (A.I.) and the embryo production (in vivo multiple ovulation and embryo transfer and in vitro Ovum pick-up and subsequent in vitro production). Ultrasound (B-Mode, color Doppler) and thermography could represent two of the diagnostic tools applicable during the peri-ovulatory period evaluating the follicular developmental synchrony. Unfortunately, we have not information on the decrease of insemination dose considering the follicular development homogeneity. In terms on number of sperm per insemination dose, the only technical approach which could optimize the parameters were rarely applied at field level and consider the ultrasonography or, above all, endoscopic trans-vaginal intra-follicular or intra-ampullar artificial insemination. In these last mentioned reproductive technologies and probable reproductive management tools for ARTs application we have only suggestions to increase the efforts to identify the insemination dose volume, sperm type, number and quality.

Five publications related to the Research Topic for the candidate interview

1. López-Gatiusa, F., Hunterb, R.H.F. Intrafollicular insemination for the treatment of infertility in the dairy cow. Theriogenology Volume 75, Issue 9, June 2011, Pages 1695-1698.

2. Radefeld, K., Papp, S., Havlicek, V., Morrell, J.M., Brem, G., Besenfelder, U. Endoscopy-mediated intratubal insemination in the cow - Development of a novel minimally invasive AI technique. Theriogenology.Volume 115, 15 July 2018, Pages 117-123.

2. Lamy, J., Corbin, E., Blache, M.-C., Garanina, A.S., Uzbekov, R. Mermillod, P, Saint-Dizier, M.Steroid hormones regulate sperm–oviduct interactions in the bovine. Reproduction Volume 154, Issue 4, 2017, Pages 497-508

2. Hunter, R.H.F. Modified insemination procedure in cattle: Deep uterine deposition of smaller numbers of frozen-thawed or sex-selected spermatozoa. (2000) Revue de Medecine Veterinaire, 151 (3), pp. 187-196.

2. Besenfelder, U., Brem, G. Tubal transfer of bovine embryos: A simple endoscopic method reducing long-term exposure of in vitro produced embryos. (1998) Theriogenology, 50 (5), pp. 739-745.